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OsmiRNA156 is a free miRNA that cleaves the mRNA transcript of the OsSPL14 gene. PCR amplification was carried out using PROMEGA kit Madison Company USA. PCR master mix was prepared for a total of 24 samples having18 μl for each sample. The individual sample reaction contains 12.24μl of water 2Mm MgCl2, taq buffer of 0.4 μl. 0.2 μl for each forward and reverse primer of 20Mm 0.4 of DNTPS and 0.16 of tag polymerase. PCR conditions were set for 40 cycles set at 94°C for 2min, followed by 30 seconds 94°C, 30 sec on 55 °C and 72°C for 30 sec, and final extension of 72°C for 10 min. During the last three years, rice production has reached to a record level. During 1940–1960s, green revolution led to an expansion in creation of agro-based industry especially in devolving countries, primarily through progress in research and innovation .

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  • Morphological evaluation of genotypes showed highest number of tillers for Basmati 370, the lowest was recorded for Line 77.
  • It is a physiological process of continuous underground branching of compact node joints of the primary shoot.
  • The SPL genes are regulated by microRNA miR156, and OsSPL16 of OsmiR156 was shown to repress OsSPL16 transcription in young panicles .
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The maximum value for the variation coefficient was recorded for grain weight, and the number of grains per panicle and revealed the lowest. Importantly, all traits showed significance for scored traits, as represented in . At a price of $10 bucks a month for the Gold membership, Asiandating.com is significantly less expensive than most sites on the web.

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All the accessions carry a C allele in fragment size of 244bp. C-A allele was located on third exon of OsSPL14, which is the target site of OsmiRNA156.

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Global food security is becoming a serious challenge for rapidly growing human population owing to negative environmental conditions caused by climate change . Therefore, it is important to accelerate the productivity of crops through breeding superior varieties. Among varipus attributes, tillering is considered an essential characteristic to determine rice yield because its significant correlation with cereal production . Plants with high tillers might be useful for adapting to diverse environmental condition however, low tillers severely influence extreme http://www.philippealbor.com/colombian-mail-order-brides-has-skills-and-relationship-information/ stress conditions . In Pakistan, rice has become next staple food after wheat, and a source of foreign earning through export. From the last several decades, agriculture system of Pakistan has faced severe challenges .

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